Ireneusz Janik

Ireneusz Janik

Technical University of Lodz, Poland, M.Sc.Eng. (1995)
Technical University of Lodz, Poland, Ph.D. (2001)

Phone: (574) 631-5464
Office: 319 Radiation Research Building

Radiation Initiated Processes in Aqueous Media

Scientific Interests

Kinetics and Mechanisms of Radical Reactions

Experimental characterization of medium effects on radiolytic rates and yields.

Spectroscopy in Extreme Environments

Optical spectroscopy of water and transients in sub- and supercritical water.

Surface Structure Characterization of Proteins by Pulsed Oxidation

Hydroxyl radical footprinting is a valuable technique for studying protein structure, but care must be taken to ensure that the protein does not unfold during the labeling process due to oxidative damage.

Radiation Chemistry of Water Soluble Polymers

Characterization of radiation induced crosslinking and scission of polymers in aqueous media.

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Recent Accomplishments

Primary Radical Rates in High Temperature Water

Irek Graphic

The rates of several key reactions of primary transients in water have been determined up to supercritical temperatures.

VUV Spectroscopy in High Temperature Water and Aqueous Solutions

Setup for studies of electronic absorption of liquid water has been developed. We are interested in investigation of the nature of the blue shift in highly compressible supercritical water (SCW) where the water density can be continuously tuned from vapor to high density fluid.

Protein Footprinting

Novel method for sub-microsecond hydroxyl radical protein footprinting has been developed. This method uses a pulsed electron beam to generate a high concentration of hydroxyl radicals by radiolysis of water. The results with ubiquitin and β-lactoglobulin A, demonstrate that one sub-microsecond electron beam pulse produces extensive protein structure modifications. Highly reactive residues that are buried within the protein structure are not oxidized, indicating that the protein retains its folded structure during the labeling process. Our ability to heavily oxidize the protein without concern for oxidation-induced unfolding allows us to detect a much greater amount of oxidation than previously reported results of the same protein, allowing for higher resolution hydroxyl radical protein footprinting data.

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Selected Publications

Janik, I., T.W. Marin. "Design of an Ultrashort Optical Transmission Cell for Vacuum Ultraviolet Spectroscopy of Supercritical Fluids." Review of Scientific Instruments 86(2015): 015102. link

Kosno, K., I. Janik, M. Celuch, J. Mirkowski, J. Kisala, D. Pogocki. "The Role of pH in the Mechanisms of  •OH Radical Induced Oxidation of Nicotine." Israel Journal of Chemistry 54 (2014): 302. link

Janik, I., G.N.R. Tripathi. "The Early Events in the •OH Radical Oxidation of Dimethyl Sulfide in Water." Journal of Chemical Physics 138 (2013): 44506. link

Janik, I. G.N.R. Tripathi. "The Nature of the Superoxide Radical Anion in Water." Journal of Chemical Physics 139 (2013): 014302. link

Janik, I., T.W. Marin. "A Vacuum Ultraviolet Filtering Monochromator for Synchrotron-based Spectroscopy." Nuclear Instruments and Methods A 698 (2013): 44. link

Wu, W., K. Nuzhdin, M. Vyushkova, I. Janik, D.M. Bartels. "Comparision of Acid Generation in EUV Lithography Films of Poly (4-hrdroxystyrene) (PHS) and Noria Adamantyl Ester (noria-AD50)" Journal of Physical Chemistry B 116 (2012): 6215. link

Ptasinska, S., D. Gschliesser, P. Bartl, I. Janik, P. Scheier, S. Danifl. "Dissociative Electron Attachment to Triflates." Journal of Chemical Physics 135 (2011): 214309. link


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